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KMID : 0380620200520010040
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Korean Journal of Food Science and Technology
2020 Volume.52 No. 1 p.40 ~ p.45
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Development of glufosinate-tolerant GMO detection markers for food safety management
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Song Min-Ji
Qin Yang
Cho Youn-Sung
Park Tae-Sung
Lim Myung-Ho
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Abstract
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Over 500 genetically modified organisms (GMOs) have been developed since 1996, of which nearly 44% have glufosinate herbicide-tolerant traits. Identification of specific markers that can be used to identify herbicide-tolerant traits is challenging as the DNA sequences of the gene(s) of a trait are highly variable depending on the origin of the gene(s), plant species, and developers. To develop specific PCR marker(s) for the detection of the glufosinate-tolerance trait, DNA sequences of several pat or bar genes were compared and a diverse combination of PCR primer sets were examined using certified reference materials or transgenic plants. Based on both the qualitative and quantitative PCR tests, a primer set specific for pat and non-specific for bar was developed. Additionally, a set of markers that can detect both pat and bar was developed, and the quantitative PCR data indicated that the primer pairs were sensitive enough to detect 0.1% of the mixed seed content rate.
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KEYWORD
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herbicide-tolerant GM crops, GMOs detection markers, pat, bar, food safety
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